Measurement of isoelectric points of phospholipid exchange proteins by gel isoelectric focusing.

Isoelectric focusing of proteins in rewetted ultrathin polyacrylamide gel layers.

Ultrathin layers of polyacrylamide gel bound to glass can be washed, air-dried, and stored for at least 1 year before rewetting in ampholyte solutions for isoelectric focusing. Short-term drying affects neither fluorescent banding of the ampholytes (not evident in conventional gels) nor resolution of complex protein mixtures while prolonged storage seems to have no deleterious effects. Layers a...

Isoelectric focusing.

Isoelectric points of multi-domain proteins

Although the distribution of protein isoelectric points is multi-modal, large proteins show isoelectric points less variable than small proteins and their isoelectric points tend to converge to a unique value, close to the pH of the milieu in which the proteins are functional, as far as the protein dimension increases. This study demonstrates that large proteins, which contain more than a singl...

Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing.

Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants of St. Louis encephalitis, Japanese encephalitis, and dengue virus envelope an...

Analysis of urinary proteins in urolithiasis by isoelectric focusing using ultra-thin-layer gel.

greater in the mutant ( P < 0.00 1 ) and remained constant with age. The heart was not affected. These results indicate that the myotonic mutation has a signficant effect on the total glucose-6-phosphatase activities of skeletal muscle and kidney, but whereas the former is raised the latter is lowered. There is a small effect on the liver and no effect on the heart. Myotonic dystrophy is known ...